鸡Piwil1基因启动子区转录调控元件的初步分析

doi:10.11843/j.issn.0366-6964.2013.09.003

Abstract

Abstract:

The aim of this study was to identify the active control area of the chicken Piwil1 gene core promoter, to predict the binding sites of the transcription factor, and to investigate the effect of the NF-Y binding sites on the chicken Piwil1 gene core promoter activity. A series of promoter deletion mutants were directly subcloned into pGL3-Basic vector. The transcriptional activity of the recombinant plasmids was detected by Dual-Glo^® Luciferase Assay System after transfecting COS-7 and GC-1 cells. In the deletion analysis of Piwil1 promoter, the core promoter essential for transcriptional activation was identified. With point mutations and Dual-Glo^® Luciferase Assay System，the effect of the NF-Y binding site on the chicken Piwil1 gene core promoter activity were identified. The activity of the -1 377--268 bp region in COS-7 and GC-1 cells were different, while the activity of -221-+1 bp region was almost disappeared. Data analysis demonstrated that there was the NF-Y binding site in -268--221 bp region. By point mutations, the activity was decreased significantly. Therefore, NF-Y site might has an important effect on Piwil1 gene promoter activity. NF-Y is an important regulatory element in Piwil1 core promoter. Furthermore, the results provide the foundation for analyzing the promoter activity and transcriptional regulation mechanism.

CLC Number:

S831.2

XIA Ming-xiu, CHANG Guo-bin, CHEN Rong, ZHAI Fei, DAI Ai-qin, MA Teng, WANG Hong-zhi, XU Lu, CHEN Jing, LIU Lu, CHEN Guo-hong. Preliminary Analysis of Transcription Regulation Elements in the Promoter Region of Piwil1 Gene in Chicken[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(9): 1349-1354.

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Preliminary Analysis of Transcription Regulation Elements in the Promoter Region of Piwil1 Gene in Chicken

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